Journal: Nature chemical biology

Article Title: Selective Inhibitors of mTORC1 Activate 4EBP1 and Suppress Tumor Growth

doi: 10.1038/s41589-021-00813-7

Figure Lengend Snippet: m a, Cell viability (CTG assay) of MCF-7 cells incubated with increasing concentrations of indicated compounds for 72 hours. Cell viability data are expressed as percent of CTG signal normalized to signal at time zero (T0) (i.e. before compound addition). Data are the mean of n=4 experiments, with error bars representing SEM. b, IncuCyte® quantification of caspase 3/7 activity in MCF-7 cells treated with increasing concentrations of indicated inhibitors for 24 hours. Data are an average of n=5 experiments each done in technical duplicates, with error bars representing SEM. c, Immunoblot analysis of whole cell lysates from MCF-7 cells incubated with indicated compounds (representative of at least n=2 independent experiments). d, Immunoblot analysis of whole cell lysates from BT474 cells incubated with indicated compounds (representative of at least n=2 independent experiments).

Article Snippet: IncuCyte® proliferation and apoptosis assays Long-term proliferation of MDA-MB-468 cells (4,000 cells/well) exposed to compounds was assessed by collecting phase-contrast images every two hours (IncuCyte, Essen BioScience).

Techniques: CTG Assay, Incubation, Activity Assay, Western Blot